phosphorylated serine  (Chem Impex International)

Journal: FEMS microbiology ecology

Article Title: Spatial heterogeneity of dechlorinating bacteria and limiting factors for in situ trichloroethene dechlorination revealed by analyses of sediment cores from a polluted field site.

doi: 10.1111/j.1574-6941.2009.00820.x

Figure Lengend Snippet: Fig. 2. SSCP profiles of PCR-amplified partial bacterial 16S rRNA genes from incubated sediment slurries (enrichment cultures) with TCE as an electron acceptor and pyruvate as an electron donor and carbon source. Bands selected for DNA sequencing are indicated by arrows. Sequence information is given in Table 2. Lane R shows reference strains Desulfitobacterium strain PCE-S (A), Desulfuromonas chloroethenica (B), Dehalobacter restrictus (C) and Dehalococcoides ethenogenes 195 (D). M, lane with SSCP markers; NC, negative control. Lanes indicated with E (a) show bacterial community profiles of enrichment cultures with complete dechlorination of TCE (formation of ethene), and those indicated with D (b) of enrichment cultures with an incomplete dechlorination (formation of cDCE; D2, D4–D6, D8–D10) or no dechlorination (D1, D3, D7).

Article Snippet: Positive controls used in this study were genomic DNA of Dehalococcoides ethenogenes strain 195 (kindly provided by Ivonne Nijenhuis, Helmholtz-Zentrum für Umweltforschung UFZ, Leipzig, Germany), Desulfitobacterium strain PCE-S, Dehalobacter restrictus (DSMZ 9455) and Desulfuromonas chloroethenica (DSMZ 12431), the latter two purchased from DSMZ (Braunschweig, Germany).

Techniques: Incubation, DNA Sequencing, Sequencing, Negative Control